The effect of ten weeks of forced swimming training with different time, on the expression of cholesterol reverse transport genes in the liver tissue of male rats
Poster Presentation
Paper ID : 1040-SSRC (R1)
Authors
1گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه دامغان، دامغان، ایران
2گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه بوعلی سینا، همدان، ایران
Abstract
Background and Aim: The process of reverse transfer of cholesterol includes collecting extra cholesterol from the surrounding tissues and returning it to the liver to form bile, which is affected by exercise training of different durations.
Materials and Methods: The 18 male rats (210±20 g) were randomly divided into three groups of six: control, medium and long-term swimming. The training groups swam in 32° water, five days/week for ten weeks. In each session, the medium group swam for one hour, and for the long group each week, was added 30 minutes to their swimming time, so that from the fifth to the tenth week, the rats swam for three hours in each session. One day after the end of the training protocol, the rats were anesthetized, and was separated the left lobe of the liver. In order to investigate the expression values of genes affecting the reverse transport of cholesterol, was used the Real-time PCR method by Master Mix Ampliqon, and DNA synthesis kit made in Denmark. Differences were determined by One-Way ANOVA method, and comparison of groups was determined by Tukey's post hoc test with p<0.05.
Results: The expression values of ABCA1 and APOA1 genes increased significantly compared to the control group (p=0.04), and there was a significant increase in the APOB gene compared to the control group (p=0.003), and compared to the medium-term group (p=0.009).
Conclusion: It seems that long-term exercise compared to medium-term has a greater effect on the process of reverse transfer of cholesterol in liver.
Materials and Methods: The 18 male rats (210±20 g) were randomly divided into three groups of six: control, medium and long-term swimming. The training groups swam in 32° water, five days/week for ten weeks. In each session, the medium group swam for one hour, and for the long group each week, was added 30 minutes to their swimming time, so that from the fifth to the tenth week, the rats swam for three hours in each session. One day after the end of the training protocol, the rats were anesthetized, and was separated the left lobe of the liver. In order to investigate the expression values of genes affecting the reverse transport of cholesterol, was used the Real-time PCR method by Master Mix Ampliqon, and DNA synthesis kit made in Denmark. Differences were determined by One-Way ANOVA method, and comparison of groups was determined by Tukey's post hoc test with p<0.05.
Results: The expression values of ABCA1 and APOA1 genes increased significantly compared to the control group (p=0.04), and there was a significant increase in the APOB gene compared to the control group (p=0.003), and compared to the medium-term group (p=0.009).
Conclusion: It seems that long-term exercise compared to medium-term has a greater effect on the process of reverse transfer of cholesterol in liver.
Keywords