The effect of eight weeks of High-Intensity Interval Training and caloric restriction on oxidative stress and antioxidant indices of aged male Wistar rats.
Poster Presentation
Paper ID : 1052-SSRC
Authors
1University of Birjand
2Ferdowsi University
3University of Birjand
Abstract
Background: Aging can be considered a natural physiological and biological process that occurs in all living organisms and is associated with a decline in body function and structure. Probably, the interactions of reactive species and endogenous antioxidants and creation of oxidative stress indicators are the main causes of aging problems. This study aimed to compare the effect of eight weeks of high intensity interval training (HIIT) and caloric restriction on oxidative stress and antioxidant indices of muscle tissue of elderly male Wistar rats.
Methods: The experimental sample of this study consisted of 32 male Wistar rats (age 23 months, weight 390±12 g) that were randomly divided into four groups: HIIT, caloric restriction(CR), HIIT with caloric restriction(CR+HIIT) and control. The eight-week HIIT program was performed with a frequency of 5 sessions per week, which included running on a treadmill at a speed of 36m/min for two minutes and active rest at a speed of 20 m/min for three minutes, until the end of the training protocol, the intervals increased from 4 to 9. In the first week, CR, CR+ HIIT groups were received 20% and then 30% less than the daily intake compared to the control group. After 48 hours from the last training session, the Gastrocnemius muscle was carefully separated and placed in liquid nitrogen, then frozen at -80°C. The levels of SOD, CAT, MDA and Thiol were measured by Spectrophotometric method. Shapiro-Wilk test was used to check the normality of data distribution. One-way analysis of variance and Bonferroni's post hoc test were used to investigate intergroup changes, and hypotheses were tested statistically at P < 0.05.
Results: The results showed that HIIT led to a significant increase in Thiol levels and a significant decrease in MDA (p < 0.05). However, calorie restriction increased CAT, Thiol, and SOD levels and decreased MDA levels (p < 0.05). 0.05) Also, HIIT combined with caloric restriction did not change CAT values, increased SOD and Thiol values, and decreased MDA (p < 0.05).
Conclusion: This research showed that HIIT and calorie restriction for eight weeks strengthen the antioxidant system in muscle tissue.
Methods: The experimental sample of this study consisted of 32 male Wistar rats (age 23 months, weight 390±12 g) that were randomly divided into four groups: HIIT, caloric restriction(CR), HIIT with caloric restriction(CR+HIIT) and control. The eight-week HIIT program was performed with a frequency of 5 sessions per week, which included running on a treadmill at a speed of 36m/min for two minutes and active rest at a speed of 20 m/min for three minutes, until the end of the training protocol, the intervals increased from 4 to 9. In the first week, CR, CR+ HIIT groups were received 20% and then 30% less than the daily intake compared to the control group. After 48 hours from the last training session, the Gastrocnemius muscle was carefully separated and placed in liquid nitrogen, then frozen at -80°C. The levels of SOD, CAT, MDA and Thiol were measured by Spectrophotometric method. Shapiro-Wilk test was used to check the normality of data distribution. One-way analysis of variance and Bonferroni's post hoc test were used to investigate intergroup changes, and hypotheses were tested statistically at P < 0.05.
Results: The results showed that HIIT led to a significant increase in Thiol levels and a significant decrease in MDA (p < 0.05). However, calorie restriction increased CAT, Thiol, and SOD levels and decreased MDA levels (p < 0.05). 0.05) Also, HIIT combined with caloric restriction did not change CAT values, increased SOD and Thiol values, and decreased MDA (p < 0.05).
Conclusion: This research showed that HIIT and calorie restriction for eight weeks strengthen the antioxidant system in muscle tissue.
Keywords