The effect of high intensity interval training and vitamin D intake on liver tissue enzymes in obese rats
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شناسه دیجیتال (DOI): 10.22089/ssrc.2024.4121
کد مقاله : 1065-SSRC
نویسندگان
Department of Exercise Physiology, Ilam Branch, Islamic Azad University, Ilam, Iran
چکیده
Background & Objective: Studies have shown the benefits of vitamin D on liver health. Also, it has been reported that there is an association between vitamin D3 deficiency and the incidence of liver disease. However, there is limited information about the simultaneous effect of vitamin D intake and exercise training on liver function indices. The purpose of this study was to investigate the effect of high intensity interval training (HIIT) and vitamin D intake on the level of aspartate aminotransferase (AST) and alanine amino transferase (ALT) enzymes in the liver tissue of obese rats.
Methodology: In this experimental study, 24 adult male Wistar rats (128 ± 32 gr) were obese with high-fat diet (including 40% cholesterol) for 14 weeks and then were randomly divided into three groups: Control, HIIT+ placebo and HIIT+ vitamin D. The amount of 10-20 thousand units of vitamin D were injected subcutaneously into rats in HIIT+ vitamin D group. Training program performed with 3- 7 sets, 31- 55m/min, 5 days a week for 12 weeks. After the last training session liver tissue was extracted and AST and ALT level measured by ELISA method.
Findings: AST and ALT enzymes level in the HIIT+ placebo and HIIT+ vitamin D were significantly lower than the control group (both; p=0.0001). Also, AST and ALT enzyme levels in the HIIT+ vitamin D group were significantly lower than the HIIT+ placebo group (p=0.0001).
Conclusion: Regarding on the results, it seems that vitamin D intake can attenuate the levels of liver enzymes and help to liver tissue health in obese rats by enhancing the effects of HIIT. Therefore, it is suggested that intake of vitamin D along with HIIT should be considered for prevent and treat the damage caused by obesity on the liver tissue. However due to the limited data in this field, more studies are needed to better understand the effect of vitamin D intake and exercise training on liver enzyme levels.
Methodology: In this experimental study, 24 adult male Wistar rats (128 ± 32 gr) were obese with high-fat diet (including 40% cholesterol) for 14 weeks and then were randomly divided into three groups: Control, HIIT+ placebo and HIIT+ vitamin D. The amount of 10-20 thousand units of vitamin D were injected subcutaneously into rats in HIIT+ vitamin D group. Training program performed with 3- 7 sets, 31- 55m/min, 5 days a week for 12 weeks. After the last training session liver tissue was extracted and AST and ALT level measured by ELISA method.
Findings: AST and ALT enzymes level in the HIIT+ placebo and HIIT+ vitamin D were significantly lower than the control group (both; p=0.0001). Also, AST and ALT enzyme levels in the HIIT+ vitamin D group were significantly lower than the HIIT+ placebo group (p=0.0001).
Conclusion: Regarding on the results, it seems that vitamin D intake can attenuate the levels of liver enzymes and help to liver tissue health in obese rats by enhancing the effects of HIIT. Therefore, it is suggested that intake of vitamin D along with HIIT should be considered for prevent and treat the damage caused by obesity on the liver tissue. However due to the limited data in this field, more studies are needed to better understand the effect of vitamin D intake and exercise training on liver enzyme levels.
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