The effects of six weeks of high-intensity interval training and Q10 supplementation on the levels of NF-KB and MuRF-1 in the soleus muscle of elderly rats
پذیرفته شده برای ارائه شفاهی
شناسه دیجیتال (DOI): 10.22089/ssrc.2024.4128
کد مقاله : 1075-SSRC (R1)
نویسندگان
1شیراز. بلوار دانشجو، میدان ارم، دانشگاه شیراز، دانشکده علوم تربیتب و روانشناسی، بخش علوم ورزشی
2شیراز. بلوار دانشجو، میدان ارم، دانشگاه شیراز، دانشکده علوم تربیتی و روانشناسی، بخش علوم ورزشی
3شیراز. بلواردانشجو، میدان ارم، دانشگاه شیراز، دانشکده علوم تربیتی و روانشناسی، بخش علوم ورزشی
چکیده
Background
Muscle atrophy is a common condition among elderly individuals and can lead to increased morbidity and mortality. Understanding the mechanisms involved in muscle atrophy during aging can help in developing better strategies to prevent or decrease muscle atrophy in aging. oxidative stress and molecular inflammation play crucial roles in age-related skeletal muscle atrophy. Therefore, this research aimed to investigate the effects of six weeks of high-intensity interval training and Q10 supplementation on the levels of NF-KB and MuRF-1 in the soleus muscle of elderly rats.
Method
The study used forty-five elderly Wistar rats, with an age range of 18-21 months and a weight of 300.15±20g. The rats were given a week to familiarize themselves with the environment and the training before being randomly divided into four groups: control, HIIT, Q10, and HIIT + Q10. They then performed incremental training on a treadmill for six weeks, with three sessions per week. For the Q10 group, Q10 was administered daily for six weeks as an oral supplement via gastric tube at a dose of 200 mg/kg body weight. NF-KB and MuRF-1 levels were analyzed by Western blot methods in the soleus muscle. The data was tested for normality and homogeneity of variances using the Shapiro-Wilk test. Differences between groups were evaluated by one-way analysis of variance test. Statistical significance was set at P≤0.05.
Results
The results of a 6-week study showed a decrease in the levels of MuRF-1 and NF-KB proteins. The group that did high-intensity interval training along with Q10 supplements showed a significant decrease in NF-KB protein levels compared to the control group (p=0.008). Additionally, both the HIIT and HIIT + Q10 groups demonstrated a significant reduction in the levels of MuRF-1 (p=0.02 and p=0.01, respectively).
Conclusion
The findings suggest that combining six weeks of HIIT with Q10 supplementation could reduce inflammation and muscle protein breakdown in elderly rats. Further research is required to validate the results and investigate the long-term effects of these interventions. By understanding their benefits and limitations, we can encourage healthy aging and improve the overall well-being of older individuals.
Muscle atrophy is a common condition among elderly individuals and can lead to increased morbidity and mortality. Understanding the mechanisms involved in muscle atrophy during aging can help in developing better strategies to prevent or decrease muscle atrophy in aging. oxidative stress and molecular inflammation play crucial roles in age-related skeletal muscle atrophy. Therefore, this research aimed to investigate the effects of six weeks of high-intensity interval training and Q10 supplementation on the levels of NF-KB and MuRF-1 in the soleus muscle of elderly rats.
Method
The study used forty-five elderly Wistar rats, with an age range of 18-21 months and a weight of 300.15±20g. The rats were given a week to familiarize themselves with the environment and the training before being randomly divided into four groups: control, HIIT, Q10, and HIIT + Q10. They then performed incremental training on a treadmill for six weeks, with three sessions per week. For the Q10 group, Q10 was administered daily for six weeks as an oral supplement via gastric tube at a dose of 200 mg/kg body weight. NF-KB and MuRF-1 levels were analyzed by Western blot methods in the soleus muscle. The data was tested for normality and homogeneity of variances using the Shapiro-Wilk test. Differences between groups were evaluated by one-way analysis of variance test. Statistical significance was set at P≤0.05.
Results
The results of a 6-week study showed a decrease in the levels of MuRF-1 and NF-KB proteins. The group that did high-intensity interval training along with Q10 supplements showed a significant decrease in NF-KB protein levels compared to the control group (p=0.008). Additionally, both the HIIT and HIIT + Q10 groups demonstrated a significant reduction in the levels of MuRF-1 (p=0.02 and p=0.01, respectively).
Conclusion
The findings suggest that combining six weeks of HIIT with Q10 supplementation could reduce inflammation and muscle protein breakdown in elderly rats. Further research is required to validate the results and investigate the long-term effects of these interventions. By understanding their benefits and limitations, we can encourage healthy aging and improve the overall well-being of older individuals.
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