Investigating the changes of TREM2 in an experimental model of vascular Dementia following the exercise-induced adaptation
Poster Presentation
Paper ID : 1141-SSRC
Authors
1M.Sc. in Exercise Physiology, Department of Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
2Assistant Professor of Motor Development, Department of Sport Science, Faculty of Humanities, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
Abstract
Background: Dementia is the most severe form of cognitive-vascular impairment that seriously disrupts patients' daily lives. Triggering receptor expressed on myeloid cells 2(TREM2)has recently been identified as a new biomarker that reflects microglial activation and innate immune response in VaD deficit. Considering that most of the studies have noticed the effect of physical activity on the treatment and rehabilitation of patients after suffering from VaD, the present study aims to investigate the preventive effect of endurance training and dual cognitive-motor task on the TREM2 expression in rats with vascular dementia.
Methods: The present study was carried out with a post-test design with a control group. 40 elderly male Wistar rats were randomly divided into 4 groups: healthy control,dementia,endurance training+dementia,and dual cognitive-motor task+dementia. The rats in the endurance training group ran on a treadmill for 8 weeks, and the cognitive-motor dual task was swimming the Morris water maze. The rats in the control group and the dementia group went through 8 weeks without any training. For creating a model of vascular dementia, the Common Carotid artery was blocked using clamps for 30 minutes. Moreover, the neurological deficit was scored with the Bederson rating system. In this stage, after hippocampal extraction and for measuring TREM2 was used western blot method. A one-way analysis of variance was used to test research hypotheses, and in case of significance, Bonferroni's post hoc test was used to compare pairs of groups. Results: The results show that following VaD after a period of endurance and cognitive-motor Dual-task training, the amount of TREM2 has increased significantly among rats with VaD compared to the control group(p=0.0001). On the other hand, the amount of TREM2 in the two training groups has decreased compared to the VaD(p=0.0001).
Conclusion: The present study showed a significant increase in TREM2 levels after a period of endurance training and cognitive-motor dual-task training compared to the control group, meanwhile this amount was lower than the dementia group that had no training experience. This level of TREM2 concentration is expected to prevent and protect cognitive and functional decline caused by neurodegenerative dementia including VaD.
Methods: The present study was carried out with a post-test design with a control group. 40 elderly male Wistar rats were randomly divided into 4 groups: healthy control,dementia,endurance training+dementia,and dual cognitive-motor task+dementia. The rats in the endurance training group ran on a treadmill for 8 weeks, and the cognitive-motor dual task was swimming the Morris water maze. The rats in the control group and the dementia group went through 8 weeks without any training. For creating a model of vascular dementia, the Common Carotid artery was blocked using clamps for 30 minutes. Moreover, the neurological deficit was scored with the Bederson rating system. In this stage, after hippocampal extraction and for measuring TREM2 was used western blot method. A one-way analysis of variance was used to test research hypotheses, and in case of significance, Bonferroni's post hoc test was used to compare pairs of groups. Results: The results show that following VaD after a period of endurance and cognitive-motor Dual-task training, the amount of TREM2 has increased significantly among rats with VaD compared to the control group(p=0.0001). On the other hand, the amount of TREM2 in the two training groups has decreased compared to the VaD(p=0.0001).
Conclusion: The present study showed a significant increase in TREM2 levels after a period of endurance training and cognitive-motor dual-task training compared to the control group, meanwhile this amount was lower than the dementia group that had no training experience. This level of TREM2 concentration is expected to prevent and protect cognitive and functional decline caused by neurodegenerative dementia including VaD.
Keywords