Poster Presentation

Paper ID : 1177-SSRC

¹Marand Branch, Islamic Azad University, Marand, Iran

²Aras Branch, Islamic Azad University, Jolfa,, Iran

Doxorubicin (DOX) is the most widely used potent anthracycline antibiotic in the treatment of various solid malignant tumors. Clinical use of DOX in cancer treatment is limited due to side effects, especially cardiotoxicity. Although the protective effect of common endurance training and the mechanisms involved in inhibiting DOX-induced cardiotoxicity have been well proven, but limited studies have been conducted on the protective effects of high-intensity interval training (HIIT) on DOX-induced cardiotoxicity. The aim of the current research was to investigate the protective effect of HIIT on DOX-induced cardiotoxicity by examining the expression changes of autophagy genes (Beclin1 and LC3II) in rat cardiomyocytes. Wistar male rats (n=6) were randomly divided into four groups: 1) Control, 2) DOX (20 mg/kg body weight, intraperitoneally), 3) HIIT (8 weeks of running on the treadmill, 7 sets of 4 minutes 80-90% VO2max separated by 3-minute periods 65-75% VO2max) and 4) HIIT+DOX. Immediately after the last session of HIIT, animals received a cumulative dose of DOX. And 72 hours after DOX injection, the animals were anesthetized by intraperitoneal injection of ketamine (90 mg/kg) and xylazine (10 mg/kg), and the heart was quickly removed. Evaluation of gene expression was done by RT-PCR test. The data were analyzed with ANOVA statistical tests and Tukey's post hoc test (α < 0.05). Based on the results, DOX led to a significant decrease in the expression of autophagy genes (Beclin1 and LC3II) (p<0.05). Also, HIIT alone and before DOX induction led to a significant increase in the autophagy genes expression (p<0.05). Therefore, HIIT could be a suitable protective strategy against DOX-induced cardiotoxicity by inhibiting the changes made in the autophagy process.

Autophagy; Cardiotoxicity; Doxorubicin; High-Intensity Interval Training