The effect of 8 weeks of high-intensity interval training on protein (eIF-2-α) and (TNF-α) changes by western blot method in liver tissue endoplasmic reticulum stress in Wistar rats with type 2 diabetes
Poster Presentation
Paper ID : 1256-SSRC (R1)
Authors
1PhD stu., Dept. of Professional Physical Education and Sport Science, Science, and Research Branch, Islamic Azad University, Tehran, Iran.
2Associate Prof., Dept. of Professional Physical Education and Sport Science, Science, and Research Branch, Islamic Azad University, Tehran, Iran.
3Prof, Dept. of Professional Physical Education and Sport Science, Kharazmi University, Tehran, Iran
4Associate Prof, Dept. of Professional Physical Education and Sport Science, Science, and Research Branch, Islamic Azad University, Tehran, Iran
Abstract
Background and purpose: Recent research has shown that endoplasmic reticulum stress in liver tissue plays a significant role in the development and development of type 2 diabetes. The aim of this study was to investigate the effect of 8 weeks of high-intensity interval training on endoplasmic reticulum stress of liver tissue in rats with type 2 diabetes.
Methods: For this purpose, 24 male Wistar rats with an age of 6 weeks and an average weight of approximately 200-250 grams were prepared. The "healthy control" group (8 mice) had free access to standard food and drinking water for 12 weeks. Another 16 mice were fed a high-fat diet for 4 weeks. Then we made these 16 rats diabetic with streptozotocin and placed them in two groups: "diabetic control" (continuation of a high-fat diet for 8 weeks without exercise) and "diabetic exercise" (continuation of a high-fat diet with intense intermittent exercise for 8 weeks). . 48 hours after the last exercise, the expression levels of eukaryotic initiator factor 2-alpha (eIF-2-α) and tumor necrosis factor alpha (TNF-α) in the liver tissue of all mice were measured by western blot method.
Findings: The expression of eIF2-α in the liver of rats in the "diabetic exercise" group was significantly decreased compared to the "diabetic control" group (P≤0.05). The amount of TNF-α protein in the "diabetic exercise" group showed a non-significant increase (P>0.05).
Conclusion: Intense interval training with the characteristics used in this research could not effectively change two endoplasmic reticulum stress markers of liver tissue in type 2 diabetic rats.
Methods: For this purpose, 24 male Wistar rats with an age of 6 weeks and an average weight of approximately 200-250 grams were prepared. The "healthy control" group (8 mice) had free access to standard food and drinking water for 12 weeks. Another 16 mice were fed a high-fat diet for 4 weeks. Then we made these 16 rats diabetic with streptozotocin and placed them in two groups: "diabetic control" (continuation of a high-fat diet for 8 weeks without exercise) and "diabetic exercise" (continuation of a high-fat diet with intense intermittent exercise for 8 weeks). . 48 hours after the last exercise, the expression levels of eukaryotic initiator factor 2-alpha (eIF-2-α) and tumor necrosis factor alpha (TNF-α) in the liver tissue of all mice were measured by western blot method.
Findings: The expression of eIF2-α in the liver of rats in the "diabetic exercise" group was significantly decreased compared to the "diabetic control" group (P≤0.05). The amount of TNF-α protein in the "diabetic exercise" group showed a non-significant increase (P>0.05).
Conclusion: Intense interval training with the characteristics used in this research could not effectively change two endoplasmic reticulum stress markers of liver tissue in type 2 diabetic rats.
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