Poster Presentation

Paper ID : 1269-SSRC

¹دانشکده علوم ورزشیدانشگاه اصفهان

²دانشکده علوم ورزشی دانشگاه اصفهان

³Department of exercise physiology,faculty of sport science,University of Isfahan,Isfahan,Iran

⁴پژوهشگاه رویان اصفهان

obesity is a risk factor for the progress of diseases and therefore a healthy lifestyle is crucial to prevent metabolic syndromes. This study was designed to evaluate the effect of exercise training on hepatic lipid synthesis in High-fat diet-fed C57BL/6 male mice. 21 mice were randomly divided into two groups. One group was fed a normal diet (n=7, ND), and another group was fed a high-fat diet (n=14, HFD) for 12 weeks. After the first 12 weeks, HFD mice were subjected to a glucose tolerance test and after, they were divided into two groups endurance training (EX) and prediabetes (preD). The training group trained for 45 minutes a day, 5 days a week for 10 weeks. In the termination, 24 hours before scarification, Weighing and the second glucose tolerance test were accomplished. subjects' plasma and liver were collected. In the preD group, body weight, glucose, insulin, insulin resistance, and lipid profile experienced a significant increase compared to the ND. In the EX-group, however, all the mentioned factors had a significant decrease. Denovo lipogenesis pathway was measured in groups and results indicated the Acsl3 which is responsible for acyl-CoA production was decreased by HFD, while this reduction was mitigated by exercise training. Srebp-1c as a master gene in the current pathway was elevated in the pre-D group compared to the ND group, while this elevation was attenuated in the exercise group. Fatty acid synthase increased in the HFD group compared to the ND group and training decreased Fasn gene expression. The expression of the first rate-limiting enzyme, Acc1 was also significantly increased in the HFD compared to the ND group, and as expected This increase was ameliorated in EX groups. Gpat1 which is responsible for triglyceride production was significantly upregulated in the HFD compared to the ND group. These increases were alleviated by exercise training. In general, HFD induced De-novo lipogenesis in the liver. While exercise training reversed or improved these changes. These findings show that regular exercise is an effective non-pharmacological approach for metabolic syndrome.

Obesity; Liver; lipogenesis; Endurance training; Exercise