Neuroprotective effects of pre-ischemic exercise on gene and proteins expression of some neurotrophic factors in rats
Poster Presentation
Paper ID : 1281-SSRC
Authors
1دانشکده تغذیه دانشگاه علوم پزشکی استان سمنان
2دانشگاه آزاد واحد گرمسار- گروه علوم ورزشی و تربیت بدنی
3هیئت پزشکی ورزشی شهرستان آرادان
4موسسه آموزش عالی سناباد گلبهار
Abstract
Background: Cerebral ischemia / reperfusion (I / R) causes irreversible damage, particularly in the hippocampal. Evidence suggests that exercise training may mitigate some of the adverse structural and functional consequences of an ischemic lesion in the brain. The purpose of this study was investigating the effects of preconditioning exercise on expression of neurotrophic factor (-3, NT-4, Trk-B and Trk-C) Gene and proteins expressionin hippocampal CA1 region and sensorimotor and Cognitive performance recovery after global cerebral I / R injury in a rat model of stroke. Methods: Thirty-five male albino Wistar rats (250 - 300 g) were randomly assigned to five groups including Exercise + Ischemia / Reperfusion (Ex + Is / Re), Control + Ischemia / Reperfusion (Co + Is / Re), and Sham. Rats in the exercise groups ran on a treadmill for 45 min / d for five days a week for 8 consecutive weeks. Common carotid artery occlusion (CCAO) procedure was used to induce ischemia. Cresyl violet (Nissl) and hematoxylin and eosin (H&E) stainings were used to determine apoptosis and the ladder rung walking task was used to assess functional impairments and recovery following ischemic lesion. Moreover, real-time PCR was used to examine gene expression. Results: CCAO promoted Cell death in and impaired sensorimotor and Cognitive performance in the ladder rung task. In contrast, pre-ischemic exercise considerably reduced Cell death and improved sensorimotor and Cognitive performance. In addition, NT-3, NT-4, Trk-B and Trk-C expression was significantly increased Ex + Is / Re compared to Co + Is / Re group (p<0.05). Conclusion: This study showed that pre-ischemic exercise can exert neuroprotective effects against ischemia in CA1 neurons Improve sensorimotor and Cognitive function.
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