The effect of an intense resistance activity session with glutamine supplementation on the relative expression of myogenin and myosin creatine kinase genes in fast twitch muscle fibers of adult male Wistar rats.
Poster Presentation
Paper ID : 1282-SSRC
Authors
1حق التدریس
2مدیر گروه تربیت بدنی دانشگاه آزاد دامغان
3مدرس تربیت بدنی دانشگاه آزاد زنجان
4رئیس دانشگاه آزاد اسلامی دامغان
Abstract
Abstract
Background:
Despite the importance of resistance training protocol and glutamine supplementation on hypertrophy, their effect on the expression of myogenic genes is still unknown, hence the aim of the present studyThe effect of an intense resistance training session with glutamine, myogenin, and creatine kinase-myosin supplements in non-nursing rats.
Methods: This study was following code of ethics IR. IAUMREC1401035 was followed in animal experimentation with 30 adult male Wistar rats for tow weeks. To acclimate rats, they were prepared for their surroundings and received proper nutrition and exercise. They were then randomly divided into a control group, an intense resistance training group with glutamine. 10 rats in each group participated in a climbing task from a flat ramp to a height of 1 in a resistance activity session for the study,5 m @ 85O slop.4 sets, 5 reps per set .Rats were subjected to a load regimen starting at 50% of their body weight. They rested for 30 seconds between repetitions and 2 minutes between sets. At the start of each set, the load increased by 10% of the rats body weight until they were carrying 80% by the fourth set , as previously reported(4). Gavagic powder, a glutamine supplement, was dissolved in 100cc of water and given at 5.5g/kg body weight each days. This study used real-time PCR to measure gene expression levels of Myosin Alfa Heavy Chain and motor Unit Type IIX. Data was analyzed using the relative foldchange method to the reference gene. The population,s normality was assessed via the Klomogrof-Smirnov test and variance equality test (Levin). One-way analyses of varience and Tukey,s post- hoc test were conducted to detect group difference, with a significance level of 5% (p<0.05).
Results
The gene expression results showed that myosin and myosin creatine kinase gene expression values increased significantly as a result of a high-intensity resistance training session with glutamine supplementation compared to the control group, and this value was more pronounced in the resistance training group (p>.5.) .
Conclusion
It seems that an intense resistance training session is more effective than glutamine supplementation on the increase of myogenic genes.
Background:
Despite the importance of resistance training protocol and glutamine supplementation on hypertrophy, their effect on the expression of myogenic genes is still unknown, hence the aim of the present studyThe effect of an intense resistance training session with glutamine, myogenin, and creatine kinase-myosin supplements in non-nursing rats.
Methods: This study was following code of ethics IR. IAUMREC1401035 was followed in animal experimentation with 30 adult male Wistar rats for tow weeks. To acclimate rats, they were prepared for their surroundings and received proper nutrition and exercise. They were then randomly divided into a control group, an intense resistance training group with glutamine. 10 rats in each group participated in a climbing task from a flat ramp to a height of 1 in a resistance activity session for the study,5 m @ 85O slop.4 sets, 5 reps per set .Rats were subjected to a load regimen starting at 50% of their body weight. They rested for 30 seconds between repetitions and 2 minutes between sets. At the start of each set, the load increased by 10% of the rats body weight until they were carrying 80% by the fourth set , as previously reported(4). Gavagic powder, a glutamine supplement, was dissolved in 100cc of water and given at 5.5g/kg body weight each days. This study used real-time PCR to measure gene expression levels of Myosin Alfa Heavy Chain and motor Unit Type IIX. Data was analyzed using the relative foldchange method to the reference gene. The population,s normality was assessed via the Klomogrof-Smirnov test and variance equality test (Levin). One-way analyses of varience and Tukey,s post- hoc test were conducted to detect group difference, with a significance level of 5% (p<0.05).
Results
The gene expression results showed that myosin and myosin creatine kinase gene expression values increased significantly as a result of a high-intensity resistance training session with glutamine supplementation compared to the control group, and this value was more pronounced in the resistance training group (p>.5.) .
Conclusion
It seems that an intense resistance training session is more effective than glutamine supplementation on the increase of myogenic genes.
Keywords