Effects of Interval Exercise Training on TGF-β and MMP-13 Gene Expression in the Liver of Dexamethasone-Treated Rats
Poster Presentation
Paper ID : 1357-SSRC
Authors
دانشگاه علوم پزشکی گیلان
Abstract
Abstract
Background and Objective: The immune system, an intricate network of specialized cells and molecules, plays a crucial role in defending against microorganisms. Dexamethasone, a corticosteroid drug, is frequently used in various pathologies to suppress immune activity.Of course,this drug is used in various pathological conditions to suppress the immune system and Its mechanism of action is different in different cells. This study investigated the effects of interval exercise training on the expression of the MMP-13 and TGF-β genes in the livers of rats treated with dexamethasone.
Methodology: In this experimental study, 24 eight-week-old rats were randomly divided into three groups of eight: a healthy control group, a dexamethasone-induced control group, and a group combining dexamethasone treatment with interval training. The interval training regimen consisted of 5-12 one-minute running intervals on a treadmill, with intensities ranging from 24 to 30 meters per minute, equivalent to 75-100% of maximal oxygen consumption. This training was conducted six days per week, incorporating 75-second active rest intervals, over a four-week period. Data analysis was performed using three-way analysis of variance (ANOVA), Kolmogorov-Smirnov, and Levene's tests, with a significance threshold set at P<0.05.
Findings: The results indicated that interval training significantly altered the expression of TGF-β in the liver of dexamethasone-treated rats, resulting in a decrease in expression. However, there was no significant impact on the expression of the MMP-13 gene.
Conclusion: The findings suggest that interval training may have a positive influence on the antioxidant system, potentially enhancing immune system resilience and reducing the adverse effects associated with corticosteroid drugs like dexamethasone.
Background and Objective: The immune system, an intricate network of specialized cells and molecules, plays a crucial role in defending against microorganisms. Dexamethasone, a corticosteroid drug, is frequently used in various pathologies to suppress immune activity.Of course,this drug is used in various pathological conditions to suppress the immune system and Its mechanism of action is different in different cells. This study investigated the effects of interval exercise training on the expression of the MMP-13 and TGF-β genes in the livers of rats treated with dexamethasone.
Methodology: In this experimental study, 24 eight-week-old rats were randomly divided into three groups of eight: a healthy control group, a dexamethasone-induced control group, and a group combining dexamethasone treatment with interval training. The interval training regimen consisted of 5-12 one-minute running intervals on a treadmill, with intensities ranging from 24 to 30 meters per minute, equivalent to 75-100% of maximal oxygen consumption. This training was conducted six days per week, incorporating 75-second active rest intervals, over a four-week period. Data analysis was performed using three-way analysis of variance (ANOVA), Kolmogorov-Smirnov, and Levene's tests, with a significance threshold set at P<0.05.
Findings: The results indicated that interval training significantly altered the expression of TGF-β in the liver of dexamethasone-treated rats, resulting in a decrease in expression. However, there was no significant impact on the expression of the MMP-13 gene.
Conclusion: The findings suggest that interval training may have a positive influence on the antioxidant system, potentially enhancing immune system resilience and reducing the adverse effects associated with corticosteroid drugs like dexamethasone.
Keywords