The effect of intense resistance training in hypoxia and normoxia conditions on inflammatory cytokines and myogenesis signaling in active young men.
Poster Presentation
Paper ID : 1387-SSRC
Authors
1Assistant Professor of exercise physiology, Department of Sport Sciences, Ardakan University, P.O. Box 184, Ardakan, Iran.
2Sports science undergraduate student, Department of Sport Sciences,, Ardakan University, Ardakan, Iran
Abstract
Objective: Intense resistance training under hypoxic conditions may exacerbate muscle hypertrophy, but little is known about this. Therefore, the aim of this research was to investigate the effect of intense resistance training in hypoxic and normoxic conditions on inflammatory cytokines and myogenesis signaling in active young men.
Methodology: The current research method was a semi-experimental one-blind method. For this purpose, 30 active men (age 25.36 ± 2.68 years) were randomly divided into two resistance training groups under conditions of hypoxic (O2:14%; N=15) and normoxic (O2:21%; N=15). In two consecutive weeks, the subjects performed 10 sessions of 1 leg knee extension (6 sets of 8 repetitions with 85% maximum 1 repetition, 2 minutes rest between sets) in hypoxic and normoxic conditions. Muscle biopsies were taken 20 minutes and 4 hours after exercise in the vastus lateralis muscle of the exercised and non-exercised legs. Blood samples were taken immediately, 1 and 3 hours after exercise. The data were analyzed using two-way ANOVA with repeated measures, one-way ANOVA and Bonferroni's post hoc test at a significance level of α=0.05.
Results: The results showed that resistance training in hypoxic compared to normoxic significantly increased NF-κB, STAT3, TNFα, IL-10, IL-8, IL-1β (p<0.05). Also, resistance training in hypoxic conditions compared to normoxic was associated with a significant increase in CD68. In addition, it was observed that resistance training in hypoxia conditions increased myogenesis by increasing myogenin and MRF4 mRNA levels.
Conclusion: Therefore, the results of the present research show that resistance training in hypoxia conditions can improve muscle hypertrophic response through myogenesis and muscle regeneration.
Methodology: The current research method was a semi-experimental one-blind method. For this purpose, 30 active men (age 25.36 ± 2.68 years) were randomly divided into two resistance training groups under conditions of hypoxic (O2:14%; N=15) and normoxic (O2:21%; N=15). In two consecutive weeks, the subjects performed 10 sessions of 1 leg knee extension (6 sets of 8 repetitions with 85% maximum 1 repetition, 2 minutes rest between sets) in hypoxic and normoxic conditions. Muscle biopsies were taken 20 minutes and 4 hours after exercise in the vastus lateralis muscle of the exercised and non-exercised legs. Blood samples were taken immediately, 1 and 3 hours after exercise. The data were analyzed using two-way ANOVA with repeated measures, one-way ANOVA and Bonferroni's post hoc test at a significance level of α=0.05.
Results: The results showed that resistance training in hypoxic compared to normoxic significantly increased NF-κB, STAT3, TNFα, IL-10, IL-8, IL-1β (p<0.05). Also, resistance training in hypoxic conditions compared to normoxic was associated with a significant increase in CD68. In addition, it was observed that resistance training in hypoxia conditions increased myogenesis by increasing myogenin and MRF4 mRNA levels.
Conclusion: Therefore, the results of the present research show that resistance training in hypoxia conditions can improve muscle hypertrophic response through myogenesis and muscle regeneration.
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