Changes of plasma Irisin to High intensity swimming training in male Wistar rat
Poster Presentation
Paper ID : 1433-SSRC
Authors
1دانشگاه هرمزگان
2گروه آموزشی تربیت بدنی دانشگاه صنعتی امیرکبیر
Abstract
Background: Irisin is known as a new myokine that has anti-inflammatory effects on adipocytes and macrophages. Irisin can improves the ability for phagocytosis and reduces the intensity of processes related to ROS production induced high intensity training. in addition, irisin directly suppresses pro-inflammatory cytokines and reduces inflammatory processes in activated lipopolysaccharides in cultured adipocytes. So the present study were to investigate the effect of high intensity swimming training on plasma irisin expression.
Methods: 25 male Wistar rat (age 8 weeks and weight 250 ± 30gr) after being familiarized with
the laboratory environment were randomly divided into swimming and control group. Rats swam high intensity training 3 days a week. The training protocol continued for 6 weeks. 48 hours after the last training session, rats were sacrificed and their blood samples were taken. After centrifugation the blood samples, they were sent to the laboratory to evaluate the changes in irisin gene expression.Then the Real-time PCR method was used to measure irisin gene expression method. Independent t-test was used to analyze the findings. Finally T-test was used to analyze the findings.
Results: plasma irisin levels were significantly increased in training group compared to the control group (p<001).
Conclusion: high intensity swimming training increased Irisin plasma levels in rat. Although high-intensity training increases ROS, irisin, with a decreasing effect on pro-inflammatory cytokines and an increasing effect on inflammatory cytokines, can reduce inflammatory signaling and also improve the function of macrophages. It seems that Irisin exerts its positive anti-inflammatory effects in RAW 264.7 macrophages through down-regulation of TLR4/MyD88 downstream pathways.
Methods: 25 male Wistar rat (age 8 weeks and weight 250 ± 30gr) after being familiarized with
the laboratory environment were randomly divided into swimming and control group. Rats swam high intensity training 3 days a week. The training protocol continued for 6 weeks. 48 hours after the last training session, rats were sacrificed and their blood samples were taken. After centrifugation the blood samples, they were sent to the laboratory to evaluate the changes in irisin gene expression.Then the Real-time PCR method was used to measure irisin gene expression method. Independent t-test was used to analyze the findings. Finally T-test was used to analyze the findings.
Results: plasma irisin levels were significantly increased in training group compared to the control group (p<001).
Conclusion: high intensity swimming training increased Irisin plasma levels in rat. Although high-intensity training increases ROS, irisin, with a decreasing effect on pro-inflammatory cytokines and an increasing effect on inflammatory cytokines, can reduce inflammatory signaling and also improve the function of macrophages. It seems that Irisin exerts its positive anti-inflammatory effects in RAW 264.7 macrophages through down-regulation of TLR4/MyD88 downstream pathways.
Keywords