The combined effect of Taurine and high- intensity interval training on the PI3K/AKT/PTEN pathway in NAFLD mice fed a high- fat diet
Poster Presentation
Paper ID : 1458-SSRC
Authors
1ندارم
2Faculty of Sports Sciences and Health, University of Tehran
3faculty of sports sciences and Health University of Tehran
4University of Tehran, Faculty of sport science and health
Abstract
None alcoholic fatty liver disease (NAFLD) is one of the most prevalent and complicated chronic liver diseases, which has been triggered by a high-fat diet (HFD) in the last few decades.
This study aimed to investigate the effect of Taurine supplementation combined with high-intensity interval training (HIIT) on the PI3K/AKT/PTEN pathway, including the insulin pathway in the liver, which is one of the critical reasons for progression of NAFLD caused by HFD in mice.
Main methods: Fifty-four male C57BL/6J mice were randomly divided into seven different groups: Control with a normal diet (CON), control with HFD (H), HFD+ HIIT (HT), HFD+Taurine supplementation (2.5%) (HTL), HFD+HIIT+ Taurine supplementation (2.5%) (HTTL), HFD+Taurine supplementation (5%) (HTH), HFD+HIIT+Taurine supplementation (5%) (HTTH). The exercise protocol consisted of 10 weeks, 5 days a week, and taurine supplementation was administered in water solubility throughout the entire period.
Key findings: Taurine supplementation at a lower dose, when combined with HIIT, demonstrated significant efficiency in the phosphorylation of PI3K, AKT, and PTEN expression, reversing the effects of HFD on this pathway. In addition, exercise and Taurine supplementation in two different dosages separately significantly increased the phosphorylation of AKT and alleviated the expression of PTEN. However, the combination of HIIT and higher doses of Taurine supplementation only decreased PTEN expression and didn’t effect on AKT. In summary, the combination of HIIT and a lower dose of Taurine could be more effective than HIIT and taurine intake individually in suppressing the exacerbation of NAFLD, with the most significant impact on AKT and PTEN.
This study aimed to investigate the effect of Taurine supplementation combined with high-intensity interval training (HIIT) on the PI3K/AKT/PTEN pathway, including the insulin pathway in the liver, which is one of the critical reasons for progression of NAFLD caused by HFD in mice.
Main methods: Fifty-four male C57BL/6J mice were randomly divided into seven different groups: Control with a normal diet (CON), control with HFD (H), HFD+ HIIT (HT), HFD+Taurine supplementation (2.5%) (HTL), HFD+HIIT+ Taurine supplementation (2.5%) (HTTL), HFD+Taurine supplementation (5%) (HTH), HFD+HIIT+Taurine supplementation (5%) (HTTH). The exercise protocol consisted of 10 weeks, 5 days a week, and taurine supplementation was administered in water solubility throughout the entire period.
Key findings: Taurine supplementation at a lower dose, when combined with HIIT, demonstrated significant efficiency in the phosphorylation of PI3K, AKT, and PTEN expression, reversing the effects of HFD on this pathway. In addition, exercise and Taurine supplementation in two different dosages separately significantly increased the phosphorylation of AKT and alleviated the expression of PTEN. However, the combination of HIIT and higher doses of Taurine supplementation only decreased PTEN expression and didn’t effect on AKT. In summary, the combination of HIIT and a lower dose of Taurine could be more effective than HIIT and taurine intake individually in suppressing the exacerbation of NAFLD, with the most significant impact on AKT and PTEN.
Keywords