Eight Weeks Aerobic Training Reduces Body Weight and Inflammation in the Liver and Visceral Adipose Tissue of Obese Rats
Poster Presentation
Paper ID : 1507-SSRC
Authors
پریسا (Parisa) خبیری (Khabiri) 1 , محمد رحمان (Mohammad Rahman) رحیمی (Rahimi)2 , ایرج (Iraj) رشیدی (Rashidi)3 , سید ارشاد (Seyed Ershad) ندایی (Nedaei)4
1گروه فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، ایران.
2گروه فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، ایران
3گروه علوم تشریح و بیولوژی سلولی، دانشکده پزشکی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران
4گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران
Abstract
Obesity is a major health challenge in the modern world that is linked to chronic disease and mortality. Sedentary lifestyle is an important risk factor for obesity. Sedentary lifestyle is spreading worldwide because of sedentary behaviors in the modern world. In order to achieve weight reduction in obesity conditions, various intervention strategies such as anti-obesity drugs, bariatric surgery, and lifestyle interventions are taken into consideration. The cheapest anti-inflammatory therapy is regular exercise. It has been known for many years that exercise has beneficial effects for health and accumulating evidence suggests that it has anti-inflammatory actions through several mechanisms.
We examined the effects of 8-week aerobic training (AT) on body weight and inflammatory markers including toll-like receptor 4 (TLR4) and nuclear factor-ƙB (NFƙB) in the liver and visceral adipose tissue (VAT) of obese rats.
Twenty-four male Sprague-Dawley rats were randomly assigned to normal diet (ND) (n=8) or high-fat diet (HFD) groups (n=16) and fed for 9 weeks. After 9 weeks ND group continued their normal diet, and the HFD group was randomly divided into HFD, and HFD+AT (5 days/week, running on treadmill); that their intervention continued for 8 weeks (n=8). During the experiment period, the rats were weighted every week to evaluate the body weight changes. At the end of the protocol, after 48 hours of the last intervention and 12 hours fasting, animals were anesthetized and the liver and VAT immediately removed and stored in liquid nitrogen for measurement of TLR4 and NFƙB mRNA expression via real-time PCR. The significance of differences among groups was assessed using one-way analysis of variance (one-way ANOVA) followed by the post-hoc Tukey test. Statistically significant differences were considered for p<0.05.
AT significantly decrease body weight compared to HFD (354.62±23.89 compared to 465.37±9.24) (p=0.001). Moreover, AT significantly decrease liver’s TLR4 (1.47±0.07 compared to 1.59±0.10) (p=0.033), and NFƙB (1.20±0.11 compared to 3.20±0.65) (p=0.001) in the and VAT’s TLR4 (0.90±0.06 compared to 1.36±0.10) (p=0.001), and NFƙB (0.83±0.07 compared to 1.65±0.04) (p=0.001) mRNA expression in the obese rats.
Aerobic training shows anti-inflammatory effects by decreasing TLR4 and NFƙB mRNA expression in the liver and VAT of obese rats.
We examined the effects of 8-week aerobic training (AT) on body weight and inflammatory markers including toll-like receptor 4 (TLR4) and nuclear factor-ƙB (NFƙB) in the liver and visceral adipose tissue (VAT) of obese rats.
Twenty-four male Sprague-Dawley rats were randomly assigned to normal diet (ND) (n=8) or high-fat diet (HFD) groups (n=16) and fed for 9 weeks. After 9 weeks ND group continued their normal diet, and the HFD group was randomly divided into HFD, and HFD+AT (5 days/week, running on treadmill); that their intervention continued for 8 weeks (n=8). During the experiment period, the rats were weighted every week to evaluate the body weight changes. At the end of the protocol, after 48 hours of the last intervention and 12 hours fasting, animals were anesthetized and the liver and VAT immediately removed and stored in liquid nitrogen for measurement of TLR4 and NFƙB mRNA expression via real-time PCR. The significance of differences among groups was assessed using one-way analysis of variance (one-way ANOVA) followed by the post-hoc Tukey test. Statistically significant differences were considered for p<0.05.
AT significantly decrease body weight compared to HFD (354.62±23.89 compared to 465.37±9.24) (p=0.001). Moreover, AT significantly decrease liver’s TLR4 (1.47±0.07 compared to 1.59±0.10) (p=0.033), and NFƙB (1.20±0.11 compared to 3.20±0.65) (p=0.001) in the and VAT’s TLR4 (0.90±0.06 compared to 1.36±0.10) (p=0.001), and NFƙB (0.83±0.07 compared to 1.65±0.04) (p=0.001) mRNA expression in the obese rats.
Aerobic training shows anti-inflammatory effects by decreasing TLR4 and NFƙB mRNA expression in the liver and VAT of obese rats.
Keywords