The effect of a period of moderate intensity aerobic activity on total antioxidant, malondialdehyde and glutathione in the hippocampal tissue of rats with type 2 diabetes
Poster Presentation
Paper ID : 1542-SSRC
Authors
کارشناس تربیت بدنی شرکت فولاد خوزستان
Abstract
Background:
Diabetes has many complications, one of the most important of which is oxidative stress. Oxidative stress, which is the result of an imbalance between the production of oxygen free radicals and the body's antioxidant defense capacity, is strongly related to diabetes and its complications. An increase in the production of free radicals or a decrease in the level of antioxidants may cause the cellular oxidative destruction of fatty acids with several double bonds in the structure of the cell membrane, and if this oxidative destruction begins, it will continue in a chain fashion, thus producing malondialdehyde (MDA). it is possible. Malondialdehyde is a molecule that belongs to the category of oxidants and free radicals and can be produced in mammalian cells. This situation may eventually cause cell death with widespread symptoms of the disease [20]. [20]. Malondialdehyde is a suitable indicator for determining the amount of cell membrane damage and oxidative stress [21] The synthesis of glutathione (GSH) from glutamate, cysteine and glycine is often catalyzed by two enzymes γ-glutamylcysteine synthetase (GCS) and GSH synthetase. [22]. Oxidative stress can damage many body tissues, including the hippocampus. Therefore, the purpose of this research is to investigate the effect of aerobic exercise with moderate intensity on oxidative stress indicators in rats with type 2 diabetes.
method:
24 adult male rats (245±9.4 g) with 10 weeks of age were divided into 4 groups of 6 (exercise diabetes, control diabetes, healthy exercise and healthy control). Rats of diabetic groups became diabetic through intraperitoneal injection of streptozotocin. The exercise training program included 8 weeks of moderate intensity endurance training. 24 hours after the last training session, hippocampus tissue samples were extracted (in order to measure the activity of total antioxidant enzymes, malondialdehyde and glutathione). One-way analysis of variance and Tukey's post hoc test with statistical level (P˂0.05) were used to compare between groups and significant differences between groups.
conclusion:
The research results showed that aerobic exercise with moderate intensity caused a significant change in the amount of malondialdehyde and glutathione, but it had no significant effect on total antioxidants (P˂0.05).
Diabetes has many complications, one of the most important of which is oxidative stress. Oxidative stress, which is the result of an imbalance between the production of oxygen free radicals and the body's antioxidant defense capacity, is strongly related to diabetes and its complications. An increase in the production of free radicals or a decrease in the level of antioxidants may cause the cellular oxidative destruction of fatty acids with several double bonds in the structure of the cell membrane, and if this oxidative destruction begins, it will continue in a chain fashion, thus producing malondialdehyde (MDA). it is possible. Malondialdehyde is a molecule that belongs to the category of oxidants and free radicals and can be produced in mammalian cells. This situation may eventually cause cell death with widespread symptoms of the disease [20]. [20]. Malondialdehyde is a suitable indicator for determining the amount of cell membrane damage and oxidative stress [21] The synthesis of glutathione (GSH) from glutamate, cysteine and glycine is often catalyzed by two enzymes γ-glutamylcysteine synthetase (GCS) and GSH synthetase. [22]. Oxidative stress can damage many body tissues, including the hippocampus. Therefore, the purpose of this research is to investigate the effect of aerobic exercise with moderate intensity on oxidative stress indicators in rats with type 2 diabetes.
method:
24 adult male rats (245±9.4 g) with 10 weeks of age were divided into 4 groups of 6 (exercise diabetes, control diabetes, healthy exercise and healthy control). Rats of diabetic groups became diabetic through intraperitoneal injection of streptozotocin. The exercise training program included 8 weeks of moderate intensity endurance training. 24 hours after the last training session, hippocampus tissue samples were extracted (in order to measure the activity of total antioxidant enzymes, malondialdehyde and glutathione). One-way analysis of variance and Tukey's post hoc test with statistical level (P˂0.05) were used to compare between groups and significant differences between groups.
conclusion:
The research results showed that aerobic exercise with moderate intensity caused a significant change in the amount of malondialdehyde and glutathione, but it had no significant effect on total antioxidants (P˂0.05).
Keywords