The effect of interval swimming training with resveratrol supplementation on oxidative stress factors and cell death in the liver tissue of elderly rats
Oral Presentation
Paper ID : 1568-SSRC
Authors
1دانشگاه آزاد اسلامی واحد علی آباد کتول
2دانشگاه آزاد اسلامی واحد مرودشت
Abstract
Abstract
Antioxidants are important factors for maintaining balance at the cellular level of important tissues of the body, including the liver, which by interacting with other proteins can maintain homeostasis balance and reduce cell damage. Therefore, the purpose of the present research is the effect of interval swimming training with resveratrol supplementation on oxidative stress factors and cell death in the liver tissue of elderly rats. In this research, 32 old rats aged 18±2 months with a weight of 350±20 gr were randomly divided into 4 groups: 1) control, 2) resveratrol, 3) training, and 4) resveratrol+training (8 rats in each group). Interval swimming training was performed for 6 weeks, 3 sessions per week. Real-time PCR laboratory method was used to measure GPX, SOD, BAX and Bcl-2 gene expression and ELISA laboratory method was used to measure MDA. Data analysis was done through one-way ANOVA and Tukey's post hoc test using SPSS version 26 software at a significance level of p ≥ 05. GPX, SOD and Bcl-2 gene expression levels increased after six weeks of interval swimming training with resveratrol supplementation (p≥05). BAX gene expression levels and MDA levels showed a significant decrease after six weeks of intermittent swimming training with resveratrol supplementation (p≥05). Taking resveratrol supplement and performing interval swimming training alone and in combination could increase the expression levels of GPX, SOD and Bcl-2 genes and decrease the levels of BAX gene expression and MDA levels. This shows that taking resveratrol supplements and training can be important therapeutic factors in improving oxidative damage at the level of liver tissue cells.
Antioxidants are important factors for maintaining balance at the cellular level of important tissues of the body, including the liver, which by interacting with other proteins can maintain homeostasis balance and reduce cell damage. Therefore, the purpose of the present research is the effect of interval swimming training with resveratrol supplementation on oxidative stress factors and cell death in the liver tissue of elderly rats. In this research, 32 old rats aged 18±2 months with a weight of 350±20 gr were randomly divided into 4 groups: 1) control, 2) resveratrol, 3) training, and 4) resveratrol+training (8 rats in each group). Interval swimming training was performed for 6 weeks, 3 sessions per week. Real-time PCR laboratory method was used to measure GPX, SOD, BAX and Bcl-2 gene expression and ELISA laboratory method was used to measure MDA. Data analysis was done through one-way ANOVA and Tukey's post hoc test using SPSS version 26 software at a significance level of p ≥ 05. GPX, SOD and Bcl-2 gene expression levels increased after six weeks of interval swimming training with resveratrol supplementation (p≥05). BAX gene expression levels and MDA levels showed a significant decrease after six weeks of intermittent swimming training with resveratrol supplementation (p≥05). Taking resveratrol supplement and performing interval swimming training alone and in combination could increase the expression levels of GPX, SOD and Bcl-2 genes and decrease the levels of BAX gene expression and MDA levels. This shows that taking resveratrol supplements and training can be important therapeutic factors in improving oxidative damage at the level of liver tissue cells.
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