The effect of 10 weeks of aerobic exercise after MSC transplantation on NO and VEGF levels in heart infarction model mice
Poster Presentation
Paper ID : 1604-SSRC
Authors
دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
Abstract
Introduction and purpose: The aim of this research was to investigate the effect of 10 weeks of aerobic training after MSC transplantation on NO and VEGF levels in heart infarction model mice.
Materials and methods: The subjects of this research are male Wistar rats. In this study, 60 male Wistar rats with an age range of 7-8 weeks were used. The subjects were divided into 6 groups of 10. healthy control group, patient control, sham, cell, exercise and cell + exercise. Before starting the protocol, the mice of the training group practiced for 1 week, during 5 sessions for 5 minutes at a speed of 5 m/min with zero incline, to familiarize themselves with the treadmill. The main training program was started 4 weeks after the myocardial infarction. The exercise program consisted of running on a treadmill for 50 minutes for 5 days a week. Kolmogorov-Smirnov test will be used to determine the normality of data distribution. One-way analysis of variance (ANOVA) test is used to check the changes of research indicators between groups.
Findings: The research results show that 10 weeks of aerobic exercise after MSC transplantation has a significant effect on the serum levels of VEGF and NO in heart infarction model mice. Serum levels of NO in cell + exercise group were higher than other groups (P<0.05) and serum levels of VEGF in sham group were higher than other groups. (P<0.05(
Conclusion: The results of this research show that the amount of NO increases in the cell + training groups compared to other groups, and it is the lowest in the patient control group. But VEGF values are the highest in the sham group and the lowest in the patient control group. Aerobic exercise after mesenchymal stem cell transplantation increases the levels of NO and VEGF, which are factors of angiogenesis and angiogenesis. Also, increasing the amount of VEGF and NO along with aerobic exercise increases angiogenic factors.
Materials and methods: The subjects of this research are male Wistar rats. In this study, 60 male Wistar rats with an age range of 7-8 weeks were used. The subjects were divided into 6 groups of 10. healthy control group, patient control, sham, cell, exercise and cell + exercise. Before starting the protocol, the mice of the training group practiced for 1 week, during 5 sessions for 5 minutes at a speed of 5 m/min with zero incline, to familiarize themselves with the treadmill. The main training program was started 4 weeks after the myocardial infarction. The exercise program consisted of running on a treadmill for 50 minutes for 5 days a week. Kolmogorov-Smirnov test will be used to determine the normality of data distribution. One-way analysis of variance (ANOVA) test is used to check the changes of research indicators between groups.
Findings: The research results show that 10 weeks of aerobic exercise after MSC transplantation has a significant effect on the serum levels of VEGF and NO in heart infarction model mice. Serum levels of NO in cell + exercise group were higher than other groups (P<0.05) and serum levels of VEGF in sham group were higher than other groups. (P<0.05(
Conclusion: The results of this research show that the amount of NO increases in the cell + training groups compared to other groups, and it is the lowest in the patient control group. But VEGF values are the highest in the sham group and the lowest in the patient control group. Aerobic exercise after mesenchymal stem cell transplantation increases the levels of NO and VEGF, which are factors of angiogenesis and angiogenesis. Also, increasing the amount of VEGF and NO along with aerobic exercise increases angiogenic factors.
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