The Effects of Six Weeks of High Intensity Interval Training with CoQ10 Supplementation on Levels of Superoxide Dismutase and Malondialdehyde in Soleus Muscle of Elderly Rats
Poster Presentation
Paper ID : 1741-SSRC (R1)
Authors
Shiraz University
Abstract
Introduction: Aging is the gradual loss of function in tissues and organs over time. During aging the levels of oxidative stress increase in the human body. On the other hand, using exercise methods and nutritional supplements is one of the ways to reduce oxidative stress.
Purpose: This study follows the effects of six weeks of High-Intensity Interval Training (HIIT) with Coenzyme Q10 supplementation on levels of Superoxide Dismutase (SOD) and Malondialdehyde (MDA) in the soleus muscle of elderly rats.
Methods: To follow this purpose, forty-four elderly Wistar rats (age: 18-21 months and weight: 300.15±20g), after one week of familiarization with the training environment, were randomly divided into four groups: control (C group), HIIT, Q10, and HIIT + Q10. During 6 weeks (3 sessions per week), rats performed incremental training on the animal treadmill. For the Q10 group, Q10 was administered daily for six weeks as an oral supplement by gastric tube at a dose level of 200 mg/kg body weight. The levels of SOD and MDA were analyzed by Elysa methods in the soleus muscle. For assessing the normality of data and homogeneity of variances, the Shapiro-Wilk test was applied, respectively. One-way analysis of variance test was used to evaluate differences between groups Statistical significance was set at P≤0.05.
Result: The results showed that there is a significant difference in levels of SOD between the C group and the HIIT+Q10 group (P=0.004), Also there is a significant difference in levels of SOD between Q10 group and HIIT+Q10 group (p=0.040). However, there is no significant difference in levels of MDA.
Conclusion: The results of this study illustrate that HIIT and Q10 together can increase Antioxidant capacity in aging muscle.
Purpose: This study follows the effects of six weeks of High-Intensity Interval Training (HIIT) with Coenzyme Q10 supplementation on levels of Superoxide Dismutase (SOD) and Malondialdehyde (MDA) in the soleus muscle of elderly rats.
Methods: To follow this purpose, forty-four elderly Wistar rats (age: 18-21 months and weight: 300.15±20g), after one week of familiarization with the training environment, were randomly divided into four groups: control (C group), HIIT, Q10, and HIIT + Q10. During 6 weeks (3 sessions per week), rats performed incremental training on the animal treadmill. For the Q10 group, Q10 was administered daily for six weeks as an oral supplement by gastric tube at a dose level of 200 mg/kg body weight. The levels of SOD and MDA were analyzed by Elysa methods in the soleus muscle. For assessing the normality of data and homogeneity of variances, the Shapiro-Wilk test was applied, respectively. One-way analysis of variance test was used to evaluate differences between groups Statistical significance was set at P≤0.05.
Result: The results showed that there is a significant difference in levels of SOD between the C group and the HIIT+Q10 group (P=0.004), Also there is a significant difference in levels of SOD between Q10 group and HIIT+Q10 group (p=0.040). However, there is no significant difference in levels of MDA.
Conclusion: The results of this study illustrate that HIIT and Q10 together can increase Antioxidant capacity in aging muscle.
Keywords