Effects of aerobic Training and Aloe Vera Supplementation on Cyclooxygenase-2 Pathway Correlates with VEGF in Mice with Breast Cancer
Poster Presentation
Paper ID : 1786-SSRC
Authors
1Associate Professor, Department of sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2Assistance Professor, Department of sport management, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3Member of Iran Innovation Center Council at Sport Sciences Research Institute of Iran, Tehran, Iran.
4Associate Professor, Department of sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Abstract
Background: The aim of this study was to determine effects of aerobic training and Aloe Vera supplementation on COX-2 and VEGF levels in mice with breast cancer.
Methodology: For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Aloe Vera and Aloe Vera + training. Breast cancer tumors were generated in mice by implantind. The training program comprised six weeks of swimming training accomplished in three sessions per week. Training time started with 10 minutes on the first day and increased to 60 minutes in the second week and the water flow rate was increased from 7 to 15 liters per minute at a constant rate. Aloe Vera extract at a dose of 300 mg/kg BW was administrated to rats by intraperitoneal injection. At the end of the study period, rats were anesthetized and blood samples were taken. Significant differences were concluded at p<0.05 with Kolmogorov-Smirnov and Tukey tests to analyze the data.
Results: The results showed significant increase in levels of serum. COX-2 and VEGF levels in the cancer group compared with the healthy group. Administration of Aloe Vera extract caused significant decrease in the COX-2 level in the cancer group. Also, in the training (swimming exercise) and Aloe Vera + training cancer groups, we observed significant decrease in the VEGF level as compared to controls.
Conclusion: Our results suggest that Aloe Vera and training inhibit the COX pathway and cause decrease production of prostaglandin E2. Hence administration of Aloe Vera in combination with aerobic training might synergistically improve the host milieu in mice bearing breast cancers.
Methodology: For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Aloe Vera and Aloe Vera + training. Breast cancer tumors were generated in mice by implantind. The training program comprised six weeks of swimming training accomplished in three sessions per week. Training time started with 10 minutes on the first day and increased to 60 minutes in the second week and the water flow rate was increased from 7 to 15 liters per minute at a constant rate. Aloe Vera extract at a dose of 300 mg/kg BW was administrated to rats by intraperitoneal injection. At the end of the study period, rats were anesthetized and blood samples were taken. Significant differences were concluded at p<0.05 with Kolmogorov-Smirnov and Tukey tests to analyze the data.
Results: The results showed significant increase in levels of serum. COX-2 and VEGF levels in the cancer group compared with the healthy group. Administration of Aloe Vera extract caused significant decrease in the COX-2 level in the cancer group. Also, in the training (swimming exercise) and Aloe Vera + training cancer groups, we observed significant decrease in the VEGF level as compared to controls.
Conclusion: Our results suggest that Aloe Vera and training inhibit the COX pathway and cause decrease production of prostaglandin E2. Hence administration of Aloe Vera in combination with aerobic training might synergistically improve the host milieu in mice bearing breast cancers.
Keywords