Investigating the effect of 10 weeks of high-intensity interval training with and without High-fat diet consumption on the mitochondrial biogenesis of the soleus muscle of male Wistar rats
Poster Presentation
Paper ID : 1787-SSRC (R1)
Authors
Department of Sport Science, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran
Abstract
Background: Mitochondria are essential in regulating healthy skeletal muscle metabolism, cellular respiration, oxidative stress, and calcium and energy homeostasis. Also, they perform various functions, including reactive oxygen species signaling, apoptosis, and immune signaling. It has been shown that High-Fat diet (HFD) consumption can decrease gene expression and protein levels of PGC1-α and disrupt mitochondrial biogenesis in humans and rodents. Studies have shown the positive effects of various exercise training, such as resistance and endurance training on mitochondrial biogenesis. Also, High-intensity interval training (HIIT) can induce mitochondrial biogenesis.
Aim: Few studies have examined the effects of HIIT in reducing and managing the negative effects of HFD consumption, and their results have been contradictory. The aim of the present study was to investigate the effect of HIIT training with and without HFD consumption on gene expression and protein levels of PGC1-α in the soleus muscle of male Wistar rats.
Materials and methods: Thirty-two healthy 6-week-old male Wistar rats with an average weight of 195±15 g were randomly divided into four groups: 1) Control (normal diet), 2) Control+HIIT, 3) HFD, and 4) HFD+HIIT. HIIT training consisted of 8 bouts of 2.5 minutes of running with an intensity of 90% of Maximum Running capacity (MRC) and 2.5 minutes of active rest with an intensity of 50% of MRC, three sessions per week. Also, HFD interventions were applied simultaneously in the form of free access for 10 weeks. RT-PCR and Western blot were used to investigate gene expression and protein level. Two-way ANOVA and LSD post hoc test were used to investigate the differences between groups.
Results: HFD decreased the expression of PGC1-α genes and protein (P<0.001). Results further showed that HIIT training increased the expression of PGC1-α (P=0.009) genes and protein (P=0.028).
Conclusion: The consumption of HFD leads to disruption in the biogenesis and ultimately reduces mitochondrial function. HIIT training moderates the negative effects of HFD consumption and improves mitochondrial biogenesis.
Aim: Few studies have examined the effects of HIIT in reducing and managing the negative effects of HFD consumption, and their results have been contradictory. The aim of the present study was to investigate the effect of HIIT training with and without HFD consumption on gene expression and protein levels of PGC1-α in the soleus muscle of male Wistar rats.
Materials and methods: Thirty-two healthy 6-week-old male Wistar rats with an average weight of 195±15 g were randomly divided into four groups: 1) Control (normal diet), 2) Control+HIIT, 3) HFD, and 4) HFD+HIIT. HIIT training consisted of 8 bouts of 2.5 minutes of running with an intensity of 90% of Maximum Running capacity (MRC) and 2.5 minutes of active rest with an intensity of 50% of MRC, three sessions per week. Also, HFD interventions were applied simultaneously in the form of free access for 10 weeks. RT-PCR and Western blot were used to investigate gene expression and protein level. Two-way ANOVA and LSD post hoc test were used to investigate the differences between groups.
Results: HFD decreased the expression of PGC1-α genes and protein (P<0.001). Results further showed that HIIT training increased the expression of PGC1-α (P=0.009) genes and protein (P=0.028).
Conclusion: The consumption of HFD leads to disruption in the biogenesis and ultimately reduces mitochondrial function. HIIT training moderates the negative effects of HFD consumption and improves mitochondrial biogenesis.
Keywords