The effect of 8 weeks of swimming training on CD9 and CD63 tetraspanins in azoospermia model rats.
Poster Presentation
Paper ID : 1866-SSRC
Authors
1Assistant Professor, Department of Physical Education and Sports Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.
2PhD student, Department of Physical Education and Sports Sciences, North Tehran Branch, Islamic Azad Univer-sity, Tehran, Iran.
3department of exercise physiology, Humanity Faculty, Sari Azad Islamic University
4Professor, Department of Physical Education and Sports Sciences, North Tehran Branch, Islamic Azad Universi-ty, Tehran, Iran.
Abstract
Background and Aim: Azoospermia refers to a condition in which a person has no sperm in the semen. This condition is often associated with a very low chance of fertility and sometimes even sterility. Spermatogenesis is the process by which immature spermatogonia are transformed into mature spermatozoa (sperm). The aim of this re-search was to investigate the effect of 8 weeks of swimming training on CD9 and CD63 tetraspanins in azoo-spermia model rats.
Materials and Methods: In this experimental study, 15 8-week-old rats were selected and randomly divided into 3 groups after induction of azoospermia model. Healthy, azoospermic and azoospermic + exercise were divid-ed. Swimming training was done daily for 30 minutes a day and 5 days a week for 8 weeks. Genes were measured by Real time-PCR method. One-way analysis of variance was used to analyze the data. All calculations were done using SPSS/23 statistical software and at a significant level of P≤0.05.
Results: The results showed that there is a significant difference between the research groups. Tukey's post hoc test results showed that in the healthy control group with the azoospermia model group, CD9 (P=0.0001) and CD63 gene expression (P=0.001) and the azoospermia model group with the azoospermia group and exercise CD9 (P=0.001) and CD63 (P=0.046) there is a significant difference. Also, no significant difference was observed in CD9 (P=0.267) and CD63 (P=0.086) between the healthy control group and the azoospermia and exercise group.
Conclusion: In general, the results of the current research indicate that regular aerobic exercise such as swimming helps in controlling the effects of infertility diseases by reducing the amount of tetraspinins in improving the process of spermatogenesis.
Materials and Methods: In this experimental study, 15 8-week-old rats were selected and randomly divided into 3 groups after induction of azoospermia model. Healthy, azoospermic and azoospermic + exercise were divid-ed. Swimming training was done daily for 30 minutes a day and 5 days a week for 8 weeks. Genes were measured by Real time-PCR method. One-way analysis of variance was used to analyze the data. All calculations were done using SPSS/23 statistical software and at a significant level of P≤0.05.
Results: The results showed that there is a significant difference between the research groups. Tukey's post hoc test results showed that in the healthy control group with the azoospermia model group, CD9 (P=0.0001) and CD63 gene expression (P=0.001) and the azoospermia model group with the azoospermia group and exercise CD9 (P=0.001) and CD63 (P=0.046) there is a significant difference. Also, no significant difference was observed in CD9 (P=0.267) and CD63 (P=0.086) between the healthy control group and the azoospermia and exercise group.
Conclusion: In general, the results of the current research indicate that regular aerobic exercise such as swimming helps in controlling the effects of infertility diseases by reducing the amount of tetraspinins in improving the process of spermatogenesis.
Keywords