The effect of exercise training on UCP1 gene expression in visceral fat and some cardiovascular risk factors in obese rats
Poster Presentation
Paper ID : 1869-SSRC
Authors
Department of Sport Sciences, Nahavand Higher Education Complex, BU-Ali Sina Univesity, Hamedan, Iran
Abstract
Introduction: The accumulation of visceral adipose tissue is associated with metabolic disorders, including cardiovascular risk factors. Exercise training is one of the main ways to reduce these factors. The aim of this study was, the effect of exercise training on changes in UCP1 gene expression of visceral fat and some cardiovascular risk factors in obese rats.
Materials and Methods: In the present study, 18 male Wistar rats were fed with high-fat food and 6 rat were nourished a standard diet for 12 weeks. After 12 weeks, obese rats were randomly divided into 2 high intensity training groups and control group. The training groups run for 14 weeks, 5 days a week on a treadmill at a speed of 34 m/min equivalent to 85% of VO2max.
Lipid profile (TG, TC, LDL and HDL) and UCP1 gene expression changes (in fat around gonads) were measured by enzymatic method and RT-PCR respectively. The data were analyzed using independent t-tests and Pearson's correlation coefficient at the P≥0.05 level.
Results: Exercise led to an increase in visceral fat UCP1 mRNA expression in the exercise group compared to the control group (P≥0.05). The reduction of TG and TC and the increase of HDL in the exercise group were significant (P≥0.05). There was an inverse and significant relationship between UCP1 and lipid profile variables except HDL (P≥0.05).
Conclusion: The effect of exercise training on reducing the lipid profile can be due to the increased activity of the thermogenic gene of visceral fat. Inverse relationship between UCP1 mRNA and cardiovascular risk factors, can make this possibility stronger. Considering the negative and significant relationship between LDL mRNA and UCP1, it seems that a significant reduction of LDL requires more intensive and long-term training.
Materials and Methods: In the present study, 18 male Wistar rats were fed with high-fat food and 6 rat were nourished a standard diet for 12 weeks. After 12 weeks, obese rats were randomly divided into 2 high intensity training groups and control group. The training groups run for 14 weeks, 5 days a week on a treadmill at a speed of 34 m/min equivalent to 85% of VO2max.
Lipid profile (TG, TC, LDL and HDL) and UCP1 gene expression changes (in fat around gonads) were measured by enzymatic method and RT-PCR respectively. The data were analyzed using independent t-tests and Pearson's correlation coefficient at the P≥0.05 level.
Results: Exercise led to an increase in visceral fat UCP1 mRNA expression in the exercise group compared to the control group (P≥0.05). The reduction of TG and TC and the increase of HDL in the exercise group were significant (P≥0.05). There was an inverse and significant relationship between UCP1 and lipid profile variables except HDL (P≥0.05).
Conclusion: The effect of exercise training on reducing the lipid profile can be due to the increased activity of the thermogenic gene of visceral fat. Inverse relationship between UCP1 mRNA and cardiovascular risk factors, can make this possibility stronger. Considering the negative and significant relationship between LDL mRNA and UCP1, it seems that a significant reduction of LDL requires more intensive and long-term training.
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