Effect of aerobic training on miR-195 and miR-30c gene expression in Heart tissue of diabetic rats
Poster Presentation
Paper ID : 1936-SSRC
Authors
1Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
22. Ph.D. student, Exercise physiology, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
Abstract
Introduction: MicroRNAs are small non-coding RNAs that regulate gene expression after transcription. They are involved in regulating physiological processes, such as adaptation to physical activity, as well as in diseases such as type 2 diabetes. The aim of this study was to investigate the effect of aerobic training on miR-195 and miR-30c gene expression in the Heart tissue of diabetic rats.
methods: For this purpose, 24 male Wistar rats (with an average weight and standard deviation of 239±28 grams and an average age of eight weeks) were randomly divided into four groups (n=6) including: 1-healthy control, 2-healthy training, 3-Diabetic control, 4-Diabetic training. In order to induce diabetes after two weeks of adaptation to the environment, 12 rats first consumed high-fat food (60%), then Streptozotocin was injected intratracheally. The training protocol included running on a treadmill at a speed of 5-10 meters per minute for 30-60 minutes, five days a week, observing the overload principle. Real-time PCR method was used to check the expression of miR-195, miR-30c genes. One-way analysis of variance and Bonferroni post hoc tests were used at a significance level of P<0.05 through SPSSV26 software.
Results: Eight weeks of T2DM resulted in a significant reduction in miR-30c gene expression and a significant increase in miR-195 gene expression compared to healthy control group (P<0.05). Training significantly increased the expression of miR-195 gene (P=0.001) and did not reduce miR-30c gene expression compared to diabetic control group P ≥0.05.
Conclusion: It seems that some of the morphological and cellular effects of type 2 diabetes on heart tissue can be influenced by the effects of eight weeks of aerobic training on miR-195. However, it may appear that the duration or intensity of training applied in this study is still insufficient to increase miR-30c gene expression levels, and further studies are needed.
methods: For this purpose, 24 male Wistar rats (with an average weight and standard deviation of 239±28 grams and an average age of eight weeks) were randomly divided into four groups (n=6) including: 1-healthy control, 2-healthy training, 3-Diabetic control, 4-Diabetic training. In order to induce diabetes after two weeks of adaptation to the environment, 12 rats first consumed high-fat food (60%), then Streptozotocin was injected intratracheally. The training protocol included running on a treadmill at a speed of 5-10 meters per minute for 30-60 minutes, five days a week, observing the overload principle. Real-time PCR method was used to check the expression of miR-195, miR-30c genes. One-way analysis of variance and Bonferroni post hoc tests were used at a significance level of P<0.05 through SPSSV26 software.
Results: Eight weeks of T2DM resulted in a significant reduction in miR-30c gene expression and a significant increase in miR-195 gene expression compared to healthy control group (P<0.05). Training significantly increased the expression of miR-195 gene (P=0.001) and did not reduce miR-30c gene expression compared to diabetic control group P ≥0.05.
Conclusion: It seems that some of the morphological and cellular effects of type 2 diabetes on heart tissue can be influenced by the effects of eight weeks of aerobic training on miR-195. However, it may appear that the duration or intensity of training applied in this study is still insufficient to increase miR-30c gene expression levels, and further studies are needed.
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