Effect of aerobic training on TNF-α level and miR-34a gene expression in Heart tissue of diabetic rats
Poster Presentation
Paper ID : 1937-SSRC
Authors
12. Ph.D. student, Exercise physiology, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran.
2Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
Abstract
ABSTRACT
Introduction: MicroRNAs play a role in diseases such as type 2 diabetes, and training has also been recognized as a strategy to reduce inflammation and apoptosis in diabetes-related heart tissue damage. The aim of this study was to investigate the effect of aerobic training on TNF-α level and miR-34a gene expression in Heart tissue of diabetic rats.
methods: For this purpose, 24 male Wistar rats (mean weight and standard deviation of 240 g and mean age of 8 weeks) were randomly divided into four groups 1) diabetic control, 2) healthy control, 3) diabetic training and 4) healthy training. Animal food was provided in the form of free access pellets and water in a 500 ml bottle. To induce type 2 diabetes, a high-fat diet (60%) was administered for two weeks and then injection of streptozotocin in a fasting state. The training protocol included running on a treadmill with electric shock stimulation at a speed of 5-10 m/min for 30-60 minutes, five days a week with the principle of overload. After eight weeks, all rats were anesthetized with ketamine xylazine. Real-time PCR and TNF-α level were used to evaluate miR-34a gene expression. One-way ANOVA and Tukey's post hoc tests were used at a significant level of P P<0.05 through SPSSV26 software.
Results: Compared to the healthy control group, diabetic rats showed higher levels of TNF-α and miR-34a (P=0.001), indicating an increase in apoptosis. Eight weeks of aerobic training resulted in significant reductions in miR-34a (P=0.023) and TNF-α (P=0.001) compared to the diabetic control group.
Conclusion: Regular aerobic training reduced the overexpression of TNF-α, especially in heart tissue. This reduction in diabetic heart inflammation and apoptosis suggests that training provides cardiovascular benefits and should be part of standard type 2 diabetes care. Also, with an increase in miR-34a in the training group compared to the diabetic group, it seems to play a role in improving diabetes.
Introduction: MicroRNAs play a role in diseases such as type 2 diabetes, and training has also been recognized as a strategy to reduce inflammation and apoptosis in diabetes-related heart tissue damage. The aim of this study was to investigate the effect of aerobic training on TNF-α level and miR-34a gene expression in Heart tissue of diabetic rats.
methods: For this purpose, 24 male Wistar rats (mean weight and standard deviation of 240 g and mean age of 8 weeks) were randomly divided into four groups 1) diabetic control, 2) healthy control, 3) diabetic training and 4) healthy training. Animal food was provided in the form of free access pellets and water in a 500 ml bottle. To induce type 2 diabetes, a high-fat diet (60%) was administered for two weeks and then injection of streptozotocin in a fasting state. The training protocol included running on a treadmill with electric shock stimulation at a speed of 5-10 m/min for 30-60 minutes, five days a week with the principle of overload. After eight weeks, all rats were anesthetized with ketamine xylazine. Real-time PCR and TNF-α level were used to evaluate miR-34a gene expression. One-way ANOVA and Tukey's post hoc tests were used at a significant level of P P<0.05 through SPSSV26 software.
Results: Compared to the healthy control group, diabetic rats showed higher levels of TNF-α and miR-34a (P=0.001), indicating an increase in apoptosis. Eight weeks of aerobic training resulted in significant reductions in miR-34a (P=0.023) and TNF-α (P=0.001) compared to the diabetic control group.
Conclusion: Regular aerobic training reduced the overexpression of TNF-α, especially in heart tissue. This reduction in diabetic heart inflammation and apoptosis suggests that training provides cardiovascular benefits and should be part of standard type 2 diabetes care. Also, with an increase in miR-34a in the training group compared to the diabetic group, it seems to play a role in improving diabetes.
Keywords