Effect of Aerobic Training on Cellular senescence Markers of Liver in Obese Rats
Poster Presentation
Paper ID : 1943-SSRC
Authors
1گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2گروه تربیت بدنی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
Abstract
Background: Obesity is a cardiovascular risk factor. Obesity and the resulting increase in cholesterol cause cell senescence by affecting the cell cycle. Exercise training reduces systemic inflammation in obese rat by strengthening the antioxidant defense in the body. In research, it was shown that moderate intensity exercise is able to restore the damaged liver tissue to the state before the induction of aging. Considering the reduction of physiological processes due to cellular senescence and the effect of aging on liver function, it seems that physical activities have a beneficial effect on improving liver function in obese samples. However, the cellular mechanisms of exercise are not well understood. Therefore, in this research, an attempt has been made to investigate the protective effect of aerobic exercise on cellular senescence markers in the liver tissue of obese rats.
Material and Methods: In this experimental study, 27 male Wistar rats were randomly divided into three groups (n=9): Normal Diet (ND), High Fat Diet (HFD) and High Fat Diet-Training (HFDT). HFD induction was performed using a high-fat diet containing 17% protein, 43% carbohydrate and 40% fat. Aerobic exercise program including running on the treadmill with an intensity of 50-60% maximal oxygen consumption (VO2max), was performed 5 days a week for eight weeks. 48 hours after the last training session, rats were anesthetized with a combination ketamine and xylazine, and after extraction, the liver tissue was placed in a nitrogen tank and sent to the laboratory to measure p16 and p21 gene expression levels. p16 and p21 gene expression levels were measured by real-time PCR. Data were analyzed by one-way analysis of variance and Tukey post hoc test at the p0.05.
Results: Results: HFD significantly increased the expression of p16 (p=0.001) and p21 (p=0.001). There was also a significant decrease in p16 (p=0.031) and p21 (p=0.045) expression in group compared to the HFD group.
Conclusion: Aerobic training improved the expression of aging-related genes in the cardiomyocytes of HFD rats. AT as an appropriate therapeutic intervention in HFD animals, it can delay cellular aging.
Material and Methods: In this experimental study, 27 male Wistar rats were randomly divided into three groups (n=9): Normal Diet (ND), High Fat Diet (HFD) and High Fat Diet-Training (HFDT). HFD induction was performed using a high-fat diet containing 17% protein, 43% carbohydrate and 40% fat. Aerobic exercise program including running on the treadmill with an intensity of 50-60% maximal oxygen consumption (VO2max), was performed 5 days a week for eight weeks. 48 hours after the last training session, rats were anesthetized with a combination ketamine and xylazine, and after extraction, the liver tissue was placed in a nitrogen tank and sent to the laboratory to measure p16 and p21 gene expression levels. p16 and p21 gene expression levels were measured by real-time PCR. Data were analyzed by one-way analysis of variance and Tukey post hoc test at the p0.05.
Results: Results: HFD significantly increased the expression of p16 (p=0.001) and p21 (p=0.001). There was also a significant decrease in p16 (p=0.031) and p21 (p=0.045) expression in group compared to the HFD group.
Conclusion: Aerobic training improved the expression of aging-related genes in the cardiomyocytes of HFD rats. AT as an appropriate therapeutic intervention in HFD animals, it can delay cellular aging.
Keywords