Changes TNF-a Gene Expression in Hippocampus Tissue in Type 2 Diabetic Rats Following High Intensity Interval Training (HIIT) Concurrent of Thyme’s Honey Consumption
Oral Presentation
Paper ID : 1953-SSRC
Authors
1Ph.D. Student., Dept. of Professional Physical Education and Sport Science, Science, and Research Branch, Islamic Azad University, Tehran, Iran.
2Assistant Prof., Dept. of Professional Physical Education and Sport Science, Science, and Research Branch, Islamic Azad University, Tehran, Iran.
3Associate Prof., Dept. of Professional Physical Education and Sport Science, Science, and Research Branch, Islamic Azad University, Tehran, Iran.
4Associate Prof., Dept. of Professional Physical Education and Sport Science, Science, and Research Branch, Islamic Azad University, Tehran, Iran..
Abstract
Overview: Type 2 diabetes is a metabolic disorder characterized by mild and chronic inflammation. If left unmanaged, it can lead to neuroinflammation, changes in the structure and function of the hippocampus, and cognitive impairment. This study aims to investigate the potential of high-intensity interval training (HIIT) exercises and thyme honey in reducing the expression of the pro-inflammatory cytokine TNF-a gene in the hippocampus tissue of rats with type 2 diabetes.
Methodology: The study involved 36 young male Wistar rats that were fed a high-fat diet for 20 weeks. Type 2 diabetes was induced by intraperitoneal injection of 25 mg/kg STZ. The rats were divided into four groups: diabetic control (C), interval training (T), thyme honey (H), and interval training-thyme honey (TH). The T and TH groups underwent training sessions consisting of a gradual increase in the intervals and intensity levels from 80 to 95% MERT over 8 weeks (5 sessions per week). The H and TH groups received 3g/kg of diluted thyme's honey through gavage. After 24 hours from the end of the interventions, the hippocampus tissue was collected for TNF-a gene expression measurement using RT-PCR. The data were analyzed using one-way and two-factor analysis of variance, as well as Bonferroni's post hoc test, with SPSS version 22.
Results: All intervention groups showed a significant reduction in TNF-a levels compared to the diabetic control group and TH compared other intervention groups (P<0.05). Also the intervention with thyme honey exhibited the largest effect size in reducing gene expression compared to the other groups.
Conclusion: The findings suggest that HIIT training and thyme honey may effectively modulate central proinflammatory cytokines and neuroinflammation associated with diabetes through epigenetic effects. Additionally, utilizing the anti-inflammatory and antioxidant properties of honey could be a potential strategy for improving diabetes-related complications, such as cognitive impairment, in addition to the role of high-intensity exercises in glycemic control.
Methodology: The study involved 36 young male Wistar rats that were fed a high-fat diet for 20 weeks. Type 2 diabetes was induced by intraperitoneal injection of 25 mg/kg STZ. The rats were divided into four groups: diabetic control (C), interval training (T), thyme honey (H), and interval training-thyme honey (TH). The T and TH groups underwent training sessions consisting of a gradual increase in the intervals and intensity levels from 80 to 95% MERT over 8 weeks (5 sessions per week). The H and TH groups received 3g/kg of diluted thyme's honey through gavage. After 24 hours from the end of the interventions, the hippocampus tissue was collected for TNF-a gene expression measurement using RT-PCR. The data were analyzed using one-way and two-factor analysis of variance, as well as Bonferroni's post hoc test, with SPSS version 22.
Results: All intervention groups showed a significant reduction in TNF-a levels compared to the diabetic control group and TH compared other intervention groups (P<0.05). Also the intervention with thyme honey exhibited the largest effect size in reducing gene expression compared to the other groups.
Conclusion: The findings suggest that HIIT training and thyme honey may effectively modulate central proinflammatory cytokines and neuroinflammation associated with diabetes through epigenetic effects. Additionally, utilizing the anti-inflammatory and antioxidant properties of honey could be a potential strategy for improving diabetes-related complications, such as cognitive impairment, in addition to the role of high-intensity exercises in glycemic control.
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