PGC-1α activation Boosts the Swimming Training-dependent Response in Skeletal Muscle of male Wistar rats
Poster Presentation
Paper ID : 1964-SSRC
Authors
1دانشگاه صنعتی امیر کبیر
2دانشگاه هرمزگان
Abstract
Background: Inadequate physical activity is linked to many chronic diseases. However, the mechanisms that tie muscle activity to health are unclear. The peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α) controls several exercise-related aspects of muscle function. Several lines of evidence suggest that PGC-1α may also have a role in regulating inflammatory processes. Inflammatory stimuli seem to regulate the activity of PGC-1α. In this study, we investigate that in response to exercise this gene suppresses the inflammatory response and mediates the beneficial effects of exercise.
Methods: Twenty-five 8-week-old Wistar rats after being familiarized with the laboratory environment were randomly assigned to two groups: control (N=10) and swimming exercise (N=15). The swimming exercise group completed a 6-week swimming program consisting of 3 days a week and 3–10 intervals. 48 hours after the last training session, rats were sacrificed and their tissue samples was taken. The levels of PGC-1α in the Biceps muscle were assessed using the Real time-PCR method. Finally, T-test was used to analyze the findings.
Results: The findings indicated that the swimming exercise group had higher levels of PGC-1α gen in the Biceps muscle (p<001).
Conclusion: The study demonstrated that 6-week swimming training was effective in reducing muscle inflammatory and oxidative stress induced by activating the PGC-1α signaling pathway. PGC-1α regulates the expression of inflammatory mediators in skeletal muscle, which may contribute to the anti-inflammatory effects of exercise. The metabolic changes induced by exercise may lead to an increase in PGC-1α expression. It appears that PGC1α exerts its exercise-induced positive anti-inflammatory effects through upstream Ca2+, β-adrenergic, p38, AMPK signaling pathways. Hence, activation of PGC-1α and the concomitant anti-inflammatory effects by regular physical activity may serve as an important tool to attenuate or even prevent low-grade inflammation and the associated pathologies.
Methods: Twenty-five 8-week-old Wistar rats after being familiarized with the laboratory environment were randomly assigned to two groups: control (N=10) and swimming exercise (N=15). The swimming exercise group completed a 6-week swimming program consisting of 3 days a week and 3–10 intervals. 48 hours after the last training session, rats were sacrificed and their tissue samples was taken. The levels of PGC-1α in the Biceps muscle were assessed using the Real time-PCR method. Finally, T-test was used to analyze the findings.
Results: The findings indicated that the swimming exercise group had higher levels of PGC-1α gen in the Biceps muscle (p<001).
Conclusion: The study demonstrated that 6-week swimming training was effective in reducing muscle inflammatory and oxidative stress induced by activating the PGC-1α signaling pathway. PGC-1α regulates the expression of inflammatory mediators in skeletal muscle, which may contribute to the anti-inflammatory effects of exercise. The metabolic changes induced by exercise may lead to an increase in PGC-1α expression. It appears that PGC1α exerts its exercise-induced positive anti-inflammatory effects through upstream Ca2+, β-adrenergic, p38, AMPK signaling pathways. Hence, activation of PGC-1α and the concomitant anti-inflammatory effects by regular physical activity may serve as an important tool to attenuate or even prevent low-grade inflammation and the associated pathologies.
Keywords