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The effect of 8 weeks of continuous and high-intensity interval training on orexin-1 receptor expression in the nucleus accombens and regulation of voluntary physical activity behavior in male Wistar rats

Poster Presentation
DOI: 10.22089/ssrc.2024.4875
Paper ID : 2093-SSRC
Authors
1,Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University Tehran, Iran
2Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran
3Department of sport science, Faculty of social science, Imam Khomeini International University, Qazvin, Iran.
Abstract
Background: A physically active way of life is linked to improved physical and mental health, and it is crucial to create supportive measures for such lifestyles. In rodents, voluntary physical activity, an active physical behavior, may be regulated by the orexinergic system. Purpose: The present study aimed to examine the effects of work-matched high-intensity interval training (HIIT) and continuous training (CT) on orexin receptor- 1 expression in the nucleus accombens tissue and voluntary wheel running of rats. Methods: Thirty-three male Wistar rats at 8- weeks of age were randomly divided into three groups control (n=11), HIIT (n=11), and CT (n=11). After the 8-weeks training period, voluntary physical activity was measured for 1 week by a cage equipped with a running wheel that calculated revolutions per day, and levels of orexin receptor-1 in the nucleus accombens were also assessed using Western blot. The data were analyzed using Graph Pad software. Results: Our findings showed that the levels of orexin receptor-1 in the nucleus accombens after 8- weeks of HIIT and CT were significantly reduced in male Wistar rats. There were no significant differences between HIIT and CT. Moreover, the significantly increased voluntary wheel running induces the effects of work-matched HIIT and CT than control, and the alterations made were greater following HIIT than CT on the desire for physical activity but these changes were not significant. Conclusions: These results indicate that reduced orexin receptor-1 correlated with increased voluntary wheel running in male Wistar rats may be associated with regulating of voluntary physical activity behavior
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