The effect of Aerobic-Resistance Training on the Expression of miR-222 and cTnT, Cx43, Ki67 Genes, and Cardiomyocyte Proliferation in Pre-Pubertal, Young, and Old Male Rats
Poster Presentation
Paper ID : 2166-SSRC
Authors
1Professor of Exercise Physiology, Faculty of Physical Education and Sport Science, University of Guilan, Rasht, Iran
2PhD Student of Exercise Physiology, Faculty of Physical Education and Sport Science, University of Guilan, Rasht, Iran
3Assistant Professor, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran
4Associate Professor, Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Abstract
Background and purpose: The evidence indicates that exercise training can affect the heart's structure at the cellular-molecular levels. Therefore, this study aimed to investigate the effect of exercise training on miR-222 and the expression of cTnT, Cx43, Ki67 genes, and cardiomyocyte proliferation in pre-pubertal, young adult, and old male rats.
Materials and methods: The number of 30 male Wistar rats in three age groups; 2 weeks as the pre-pubertal group, 8 weeks as the young group, and 96 weeks as the old group were randomly divided into two training groups (n=5), and control (n=5). Resistance training programs (resistance ladder, 3 days per week) and aerobic (treadmill running, 3 days per week) were performed for 6 weeks. The expression of miR-222 and Cx43, cTnT, and Ki67 genes were investigated by the Real Time-PCR method. One-way analysis of variance and Tukey's post hoc test were used (P≤0.05).
Results: The amount of BrdU protein by immunohistochemical method and Ki67 gene expression was significantly higher in the training groups than in the control groups in all three age groups: pre-pubertal, young adult, and old male rats (P≤0.05). miR-222 was significantly higher in the group of trained pre-pubertal than in the control group (P=0.023). The increase in cTnT gene expression in the pre-pubertal groups (P=0.018) and old rats (P=0.015) and the increase in Cx43 gene expression in the old rats group (P=0.009) was observed.
Conclusion: Aerobic-resistance training can be an effective stimulus to increase cardiomyocyte proliferation in all three age groups pre-pubertal, young, and old.
Materials and methods: The number of 30 male Wistar rats in three age groups; 2 weeks as the pre-pubertal group, 8 weeks as the young group, and 96 weeks as the old group were randomly divided into two training groups (n=5), and control (n=5). Resistance training programs (resistance ladder, 3 days per week) and aerobic (treadmill running, 3 days per week) were performed for 6 weeks. The expression of miR-222 and Cx43, cTnT, and Ki67 genes were investigated by the Real Time-PCR method. One-way analysis of variance and Tukey's post hoc test were used (P≤0.05).
Results: The amount of BrdU protein by immunohistochemical method and Ki67 gene expression was significantly higher in the training groups than in the control groups in all three age groups: pre-pubertal, young adult, and old male rats (P≤0.05). miR-222 was significantly higher in the group of trained pre-pubertal than in the control group (P=0.023). The increase in cTnT gene expression in the pre-pubertal groups (P=0.018) and old rats (P=0.015) and the increase in Cx43 gene expression in the old rats group (P=0.009) was observed.
Conclusion: Aerobic-resistance training can be an effective stimulus to increase cardiomyocyte proliferation in all three age groups pre-pubertal, young, and old.
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