Hypoglycemic interactive effect of swimming training and creatine supplementation in high fat diet-induced obese rats is mediated through increased muscle glycogen regulator proteins
Poster Presentation
Paper ID : 2173-SSRC
Authors
فیزیولوژی ورزش، دانشکده علوم ورزش، دانشگاه بوعلی سینا،همدان، ایران
Abstract
Background: Obesity could result in glucose metabolism dysregulation. Therefore, the aim of this study was to investigate the effect of exercise training alone and in combination with creatine supplementation on glucose homeostasis, insulin efficiency and muscle glycogen regulator proteins in high fat died-induced obese rat.
Methods: 50 male wistar rats (17±170 g) were divided into two groups of 10 control (standard food) and 40 high-fat diet (HFD). After 12 weeks after induction of obesity, 10 control rats and 10 rats from HFD group were killed. Then, 30 obese rats were randomly divided into three groups: control obese, swimming training and swimming training plus creatine supplementation. Finally, rats underwent oral glucose tolerance test (OGTT) and blood samples were taken to measure glucose and insulin levels. Also, the right SOL muscle was removed to measure the protein level of GLUT4 and glycogen synthase.
Results: After 12 weeks of HFD consumption, obesity decreased glucose tolerance and insulin efficiency and increased fasting insulin and glucose in rats. Also, obesity reduced the protein levels of GLUT4 and muscle glycogen synthase. Ten weeks of training increased glucose tolerance, insulin efficiency and increased protein levels of GLUT4 and glycogen synthase. Compared with training alone, creatine supplementation combined with training synergistically increased glucose tolerance, insulin efficiency and muscle GLUT4 and GS protein levels (P<0.05).
Conclusion: Creatine supplementation increased the hypoglycemic effects of swimming exercises and these effects are probably due to higher glucose harvesting and increased levels of GLUT4 proteins and muscle glycogen synthase. Creatine supplementation resulted in increasing training hypoglycemic effects and these effects are likely mediated through further glucose uptake and increasing muscle GLUT4 and GS protein levels.
Methods: 50 male wistar rats (17±170 g) were divided into two groups of 10 control (standard food) and 40 high-fat diet (HFD). After 12 weeks after induction of obesity, 10 control rats and 10 rats from HFD group were killed. Then, 30 obese rats were randomly divided into three groups: control obese, swimming training and swimming training plus creatine supplementation. Finally, rats underwent oral glucose tolerance test (OGTT) and blood samples were taken to measure glucose and insulin levels. Also, the right SOL muscle was removed to measure the protein level of GLUT4 and glycogen synthase.
Results: After 12 weeks of HFD consumption, obesity decreased glucose tolerance and insulin efficiency and increased fasting insulin and glucose in rats. Also, obesity reduced the protein levels of GLUT4 and muscle glycogen synthase. Ten weeks of training increased glucose tolerance, insulin efficiency and increased protein levels of GLUT4 and glycogen synthase. Compared with training alone, creatine supplementation combined with training synergistically increased glucose tolerance, insulin efficiency and muscle GLUT4 and GS protein levels (P<0.05).
Conclusion: Creatine supplementation increased the hypoglycemic effects of swimming exercises and these effects are probably due to higher glucose harvesting and increased levels of GLUT4 proteins and muscle glycogen synthase. Creatine supplementation resulted in increasing training hypoglycemic effects and these effects are likely mediated through further glucose uptake and increasing muscle GLUT4 and GS protein levels.
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